FRISBI

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FRENCH INFRASTRUCTURE FOR INTEGRATED STRUCTURAL BIOLOGY

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CBS

ISOTOPIC LABELLING

The isotope labelling platform of the CBS is devoted to the large scale production of proteins uniformly or specifically enriched in stable isotopes (15N, 13C and 2H) for biomolecular NMR spectroscopy studies. The platform is offering access to standard labelling schemes,

Activities proposed by the platform:

Users have access to the facility for large scale expression of labelled proteins in E.coli, under the supervision of a qualified platform engineer.

Optimised protocols and adequate isotopically labelled materials are available to users for the following services:

• Feasibility studies and adaptation of protein over-expression in minimal and/or perdeuterated media.

• Large scale over-expression of [15N,13C,2H]-proteins,

• Training in large scale production of isotopically labelled proteins (practical experience in protein biochemistry is required).