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FRENCH INFRASTRUCTURE FOR INTEGRATED STRUCTURAL BIOLOGY

Thermal-Shift-Assay.jpg

CBS

THERMAL SHIFT ASSAY

Thermal shift assay is a thermodenaturation assay monitoring the thermal stability of proteins and investigate factors affecting this stability. This rapid and simple technique can be used in medium-throughput mode to screen optimal solubility conditions for purified macromolecules.

At the CBS, the differential scanning fluorimetry (DSF) method is available.

The optimization of proteins solubility and stability properties improves the success rate of their structural studies [1]. Changes in the thermal stability of the protein–ligand or protein-peptide complexes relative to the stability of the protein alone allow to rapidly identify promising complexes for further structural characterization and to assign functions.

Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination Vedadi et al.,Proc Natl Acad Sci. (2006) , 103, 15835-40.

Device:

Mx3005P QPCR system.