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FRENCH INFRASTRUCTURE FOR INTEGRATED STRUCTURAL BIOLOGY

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INSTRUCT CENTER - FRANCE 1 - IGBMC

BACTERIAL EXPRESSION

IGBMC Strasbourg bacterial expression platform combines (i) a large set of expression vectors for Escherichia coli and (ii) robotized protocols, which enable a broad screening of soluble protein expression through the use of multiple fusion proteins and various growth conditions.

IGBMC Strasbourg bacterial platform has also a long standing and unique expertise in protein complexes reconstitution and production by multi-expression in Escherichia coli. This technology is fully compatible with the automated protocols used for single expression.

All bacterial expression vectors provided by the platform are compatible with major cloning strategies (e.g. restriction, Gateway, SLIC).

IGBMC bacterial expression platform offers two modes of access:

  1. The platform provide the expression vectors to the external users who perform cloning at their home institutions and come to IGBMC to perform the expression assays.
  2. The external users perform cloning and expression assays at the IGBMC platform.

Support includes training and supervision, as well as help with data analysis which is especially crucial for complex reconstitution.

Comprehensive lists of single- and multi-expression vectors available at the platform can be found at:

http://igbmc.fr/Romier_Multi-Expression/

For more details see: https://www.structuralbiology.eu/update/platform/expression-in-bacteria-at-igbmc-cerbm-strasbourg