FRISBI

logo frisbi

FRENCH INFRASTRUCTURE FOR INTEGRATED STRUCTURAL BIOLOGY

In-house-X-ray-crystallography.jpg

INSTRUCT CENTER - FRANCE 1 - IGBMC

IN HOUSE-X-RAY CRYSTALLOGRAPHY

Ultra high-intensity microfocus rotating anode X-ray generator coupled with a diffractometer for collection of diffraction data from single crystals, and a BioSAXS device for collection of diffusion data from macromolecular solutions. The variable divergence on the optics, coupled with a pixel array detector, allows for the resolution of unit cell dimensions up to 600Å.

Device:

Rigaku FR-X generator coupled with Osmic VariMax HF Arc)Sec Confocal optics and a Dectris Pilatus 300K pixel array detector on the left port, and a Rigaku BioSAXS-1000 system on the right port. This generator has a focal spot size of µm and a power of 2.97kW leading to a brillance of 1.2 x 1010 photons/sec/mm2/mR2. The VariMax Arc)Sec HF optics have a divergence range of 4.8mR - 0mR (by moving the divergence slits) leading to a maximum beam size of 0.19mm.

BIBLIOGRAPHY

Structure of the Elongator cofactor complex Kti11/Kti13 provides insight into the role of Kti13 in Elongator-dependent tRNA modification. Kolaj-Robin OMcEwen AGCavarelli JSéraphin BFEBS J. 2015 Mar;282(5):819-33. doi: 10.1111/febs.13199. Epub 2015 Feb 4.