Cryo Electron Microscopy (Cryo-EM)
The EM platform is composed of:An electron microscope JEOL 1400 equipped with a Lab6 operated up to 120 keV.An electron microscope Glacios (Thermofisher) operated at 200 kVAn EM-GP2 plunger (Leica) to freeze cryo-grids.A cryogenic FIB-SEM (Zeiss), combining a focused galium ion beam with a scanning electron microscope Gemini 2 The EM platform proposes different services:Negative […]
Cryo Electron Microscopy
The cryo-electron microscopy platform comprises two cryo-electron microscopes 200 kV and 120 kV (Tecnai FEI), each equipped with a direct detection camera (K2 Gatan) and a liquid nitrogen cooled holder (Gatan 626). The 200 kV microscope is equipped with a field emission gun (FEG) and a camera that detects single electrons (Gatan K2 summit); the 120 kV has a LaB6 filament […]
Cryo Electron Microscopy (Cryo-EM)
The EM platform provides three types of services The cryo-electron microscopes, and the associated instruments, are available under conditions. Different techniques are available in the platform to allow the observation of biological samples 1. Fixation 2. Inclusion 3. Sectioning 4. Labeling and Staining 5. Observation The platform also provides access to equipments under certain conditions Scientific collaborations, that require specific developments, are […]
Cryo Electron Microscopy
The Titan Krios cryo electron microscope is the latest generation electron microscope with capacities for high-resolution data collection for both Cryo Electron Tomography (CET) experiments and Single Particle Analysis (SPA) with automated data collection. The electron source is a Field Emission Gun (FEG) that can be operated at 80keV, 100keV, 200keV or 300keV. An automatic loading […]
Cryo Electron Microscopy
The methods of X-ray crystallography and NMR spectroscopy can provide detailed information on molecular structure and dynamics. At the cellular level, optical microscopy reveals the spatial distribution and dynamics of molecules tagged with fluorophores. Electron microscopy (EM) overlaps with these approaches, covering a broad range from atomic to cellular structures. The development of cryogenic methods […]