FRISBI
FRISBI offers a range of workshops to train graduate and post-graduate students in integrated structural biology. FRISBI courses are open to french and european researchers, from academic and industry.
ReNaFoBis / FRISBI webinar
Please find below the program of the next FRISBI/ReNaFoBis webinar on May 15th at 01:00 PM Paris
Speaker: Marc Jamin, IBS, Grenoble, France
Tittle: Deciphering the mechanisms of liquid phase separation induced by rabies virus phosphoprotein
Khadeeja Mubashira, Fella Bouchama, Caroline Mas, Christine Ebel, Sylvain Prevost, Thomas Zemb, and Marc Jamin,
Rabies virus (RABV) generates membrane-less liquid organelles (Negri bodies) in the cytoplasm of its host cell, where genome transcription and replication and nucleocapsid assembly take place, but the mechanisms of their assembly and maturation remain to be explained. An essential component of the viral RNA synthesizing machine, the phosphoprotein (P), acts as a scaffold protein for the assembly of these condensates. This intrinsically disordered protein forms star-shaped dimers with N-terminal negatively charged flexible arms and C-terminal globular domains exhibiting a large dipole moment. Our study shows that in vitro self-association of RABV P drives a complex thermoresponsive phase separation with a lower critical solution temperature. Protein dimers assemble already below the saturation concentration, and condensation is driven by attractive conformation-specific interactions leading to reentrant liquid phase separation over a narrow range of salt concentration. We propose a minimal molecular model in which P can adopt three limit conformational states and the disordered N-terminal arms control the interactions between giant dipoles that is consistent with our observations.
e-mail: marc.jamin@ibs.fr
PLEASE REGISTER HERE:
https://us06web.zoom.us/webinar/register/WN_hx7hikloSpexd40swI_UnQ
BioStine
FRISBI support BioStine a site to access the basics in Structural Biology in French Biostine FR , or in English Biostine EN
Une play-list youtube dédiée à la Biologie Structurale Intégrative (Coordination: Marie-Hélène Le Du, Paris-Saclay): https://www.youtube.com/playlist?list=PLJXFANNaperht_5i-UzBTvCLAH0-CbIg6
Past webinars:
April 24 at 01:00 PM Paris
Speaker: Simon Pichard, IGBMC/CBI, Illkirch, France Tittle: Eukaryotic production and characterization of multi-protein complexes: tools and examples and Speaker: Amélie Zachayus, IGBMC, France Title: New Models, New Insights: How CRISPR-Cas9 and Live-Cell Analysis can be used to Enhance Our Understanding of Cellular Mechanisms: the example of the transcription/DNA repair factor TFIIH
March 13, 2025 Speaker: Claude Sauter, IBMC, Strasbourg, France Tittle: Microfluidic chips for biomolecular crystal growth and serial crystallography; Serena Sirigu, SOLEIL, Paris-Saclay, France Tittle: In-situ X-ray crystallography with the CRIBLEUR on the PROXIMA-2A beamline at Synchrotron SOLEIL
February 13, 2025 Speaker: Tobias Madl University of Graz, Austria Tittle "Decoding the Regulatory Role of Post-Translational Modifications with NMR Spectroscopy"
Please follow our program of ReNaFoBis-FRISBI webinars
FRISBI is involved and strongly support the French National training network in structural biology (ReNaFoBiS). Aimed to be a national PhD training network, ReNaFoBiS coordinate and organize classes, workshops and training programs for students and post-docs.
See http://www.renafobis.fr/ for more information
