TRAINING

FRISBI offers a range of workshops to train graduate and post-graduate students in integrated structural biology. FRISBI courses are open to french and european researchers, from academic and industry.

ReNaFoBis / FRISBI webinar

Please find below the program of the next FRISBI/ReNaFoBis webinar on July 10^th at 01:00 PM Paris 

Speaker:

Leandro Estrozi, IBS, Grenoble 

Title: Helical Symmetry Determination for 3D Cryo-Electron Microscopy<u5:p></u5:p>

Abstract: Determining the symmetry of helical assemblies using 3D cryo-electron microscopy is a complex and challenging task. Despite involving only two real parameters - the helical twist angle and the axial rise distance - converging to an incorrect symmetry is a well-documented issue in the field. Experts generally agree that no fully satisfactory theoretical solution exists, leaving trial-and-error as the primary approach. However, correctly identifying the symmetry can significantly improve the resolution of 3D reconstructions.
In this webinar we will show how to use the online tool HELIXPLORER (http://rico.ibs.fr/helixplorer) to rationally and visually define a finite list of plausible symmetries or parameter ranges to be tested against cryo-EM data.<u5:p></u5:p>

Speaker : Denis Chrétien, Université de Rennes<u5:p></u5:p>

Title: C- and D-type lateral interactions weaken the microtubule lattice<u5:p></u5:p>

Abstract: Tubulin subunits (alpha, beta) are usually considered to engage two types of lateral interactions within microtubules, heterotypic (a-b, b-a) or homotypic (a-a, b-b) of the A- and B-type, respectively. These lateral interactions follow left-handed, 2- to 4-start monomeric helical paths in microtubules with varying protofilament numbers. Using a combination of cryo-electron microscopy and tomography, we describe two new types of lateral interactions that are shifted about half the tubulin monomer repeat along the protofilament axis from the classical ones, giving rise to 3.5-start lateral monomer helices. The two protofilaments involved in these C- and D-type lateral interactions rotate substantially towards each other and engage weaker contacts than the A- and B-types. They allow microtubules to adopt protofilament skew angles intermediate to those observed in 3- and 4-start microtubules for a same protofilament number, consistent with a relaxation mechanism in response to high protofilament skew. They occur at a high frequency in the presence of the slowly hydrolysable GTP-analogues GDP-BeF3-, GTPgS, and GDP-AlFx, and appear at a low frequency with GMPCPP only at high tubulin concentration. They form at the onset of tubulin self-assembly in the presence of GTP to disappear rapidly at the beginning of the sigmoidal assembly phase, suggesting that they are not stable enough to remain trapped in the microtubule lattice in dynamic assembly conditions. By analogy with defects found in materials, these weaker interactions must impact microtubule's mechanical properties and their response to stress, and as a consequence their stability and dynamic properties.<u5:p></u5:p>

PLEASE REGISTER HERE:

https://us06web.zoom.us/webinar/register/WN_IH_Q_Kg5TXOhW085A0Ev0Q

 

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BioStine

FRISBI support BioStine a site to access the basics in Structural Biology in French Biostine FR , or in English Biostine EN

Une play-list youtube dédiée à la Biologie Structurale Intégrative (Coordination: Marie-Hélène Le Du, Paris-Saclay): https://www.youtube.com/playlist?list=PLJXFANNaperht_5i-UzBTvCLAH0-CbIg6

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Past webinars:

May 15^th at 01:00 PM Paris 

Speaker: Marc Jamin, IBS, Grenoble, France Tittle: Deciphering the mechanisms of liquid phase separation induced by rabies virus phosphoprotein

April 24 at 01:00 PM Paris

Speaker: Simon Pichard, IGBMC/CBI, Illkirch, France Tittle: Eukaryotic production and characterization of multi-protein complexes: tools and examples and Speaker: Amélie Zachayus, IGBMC, France Title: New Models, New Insights: How CRISPR-Cas9 and Live-Cell Analysis can be used to Enhance Our Understanding of Cellular Mechanisms: the example of the transcription/DNA repair factor TFIIH

Please follow our program of ReNaFoBis-FRISBI webinars

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FRISBI is involved and strongly support the French National training network in structural biology (ReNaFoBiS).  Aimed to be a national PhD training network, ReNaFoBiS coordinate and organize classes, workshops and training programs for students and post-docs.

See http://www.renafobis.fr/   for more information