Multi-Angle static Light Scattering (MALS) measures molar mass directly, in solution. Combined with a fractionation technique like Size Exclusion Chromatography (SEC), MALS determines absolute molar mass distributions independent of elution time and as the molecule exists in solution.
Molar mass distributions of heterogeneous samples
Analysis of aggregation and oligomeric states
Stoichiometry of macromolecular complexes
Characterisation of conjugated macromolecules (glycoproteins, PEGylated proteins, membrane proteins solubilized in detergent micelles)
Device: HPLC Ultimate 3000 (Thermo Scientific) / DAWN 8 angles light-scattering detector coupled to an Optilab differential refractometer
For more information see http://www.afmb.univ-mrs.fr/en/facility/structural-biology/biophysical-techniques/