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FRENCH INFRASTRUCTURE FOR INTEGRATED STRUCTURAL BIOLOGY

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AFMB

THERMAL SHIFT ASSAY

"nanoDSF (Tycho)"

Tycho measures protein's structural integrity or foldedness in a label-free way. The system measures the fluorescence of intrinsic tryptophan and tyrosine residues detected at both 350 nm and 330 nm as a
30 oC/minute temperature ramp is applied from 35-95 oC. It quickly reveals protein quality - presence, purity, concentration, functionality and similarity - all in one experiment.
Applications:
Test storage conditions, different batches comparison, improve buffer recipe development, confirm sample quality of crystals before running x-ray crystallography experiments, check quality of samples prior to cryo-EM

Device: Tycho NT.6 (Nanotemper)