FRISBI
NMR spectroscopy provides invaluable insights into the structural and dynamical features of biomolecular systems. It is particularly well suited to analyze multiple functional states, internal flexibility and interaction processes involving proteins at an atomic scale.
Main application field: Structure, dynamics and interactions of biomolecular systems; Analysis of small amount of small molecules (especially natural products)
Spectrometers:
ICSN: Five spectrometers (2*600 MHz, 700 MHz, 800 MHz and 950 MHz). For more details on these spectrometers, go to:
https://icsn.cnrs.fr/en/platforms/nmr
I2BC: Two spectrometers (600 MHz and 700 MHz Bruker Avance III / NEO spectrometers, equipped with TCI (1H,13C, 15N, 2H) 5mm, z-gradients cryoprobes), go to:
https://www.i2bc.paris-saclay.fr/structural-biology/nmr/
1. Polθ is phosphorylated by Polo-like kinase 1 (PLK1) to enable repair of DNA double strand breaks in mitosis. Camille Gelot, MartonTibor Kovacs, Simona Miron, Emilie Mylne, Rania Ghouil, Tatiana Popova, Florent Dingli, Damarys Loew, Josée Guirouilh-Barbat, Elaine Del Nery, Sophie Zinn-Justin, Raphael Ceccaldi. Nature (2023) doi: 10.1038/s41586-023-06506-6.
2. Unexpected binding modes of inhibitors to the histone chaperone ASF1 revealed by a foldamer scanning approach. Perrin ME, Li B, Mbianda J, Bakail M, André C, Moal G, Legrand P, Ropars V, Douat C, Ochsenbein F, Guichard G. Chem Commun (Camb) (2023) doi: 10.1039/d3cc01891a.
3. Structural transitions in TCTP tumor protein upon binding to the anti-apoptotic protein family member Mcl-1. Malard F, Sizun C, Thureau A, Carlier L, Lescop E.J Biol Chem. (2023) doi: 10.1016/j.jbc.2023.104830.
4. The Conformation of the Intrinsically Disordered N-Terminal Region of Barrier-to-Autointegration Factor (BAF) is Regulated by pH and Phosphorylation. Marcelot A, Zinn-Justin S, Cuniasse P. J Mol Biol. (2023) doi: 10.1016/j.jmb.2022.167888.
5. Exploration of the dynamic interplay between lipids and membrane proteins by hydrostatic pressure. Pozza A, Giraud F, Cece Q, Casiraghi M, Point E, Damian M, Le Bon C, Moncoq K, Banères JL, Lescop E, Catoire LJ. Nat Commun. (2022) doi: 10.1038/s41467-022-29410-5.
6. In-Cell Structural Biology by NMR: The Benefits of the Atomic Scale. Theillet FX. Chem Rev. (2022) doi: 10.1021/acs.chemrev.1c00937.
7. Di-phosphorylated BAF shows altered structural dynamics and binding to DNA, but interacts with its nuclear envelope partners. Marcelot A, Petitalot A, Ropars V, Le Du MH, Samson C, Dubois S, Hoffmann G, Miron S, Cuniasse P, Marquez JA, Thai R, Theillet FX, Zinn-Justin S. Nucleic Acids Res. (2021) doi: 10.1093/nar/gkab184.
8. Optimal anchoring of a foldamer inhibitor of ASF1 histone chaperone through backbone plasticity. Mbianda J, Bakail M, Andre C, Moal G, Perrin ME, Pinna G, Guerois R, Becher F, Legrand P, Traore S, Douat C, Guichard G, Ochsenbein F. Sci Adv. (2021) doi: 10.1126/sciadv.abd9153.