FRISBI
Tuesday, June the 25th at 11 am, we will a have a FRISBI/ReNaFoBis webinar by Florian FAESSLER (IGBMC, Illkirch)
Title: Cryo-electron tomography of filament assemblies and their organizers
Please register here: https://us06web.zoom.us/webinar/register/WN_XP6fismwQQCFNXtjA1wEUg
Summary: Cryo-electron tomography (cryo-ET) combined with subtomogram averaging allows structure determination in complex environments, such as cells, often recalcitrant to other structural biology approaches. While the achievable resolutions in most cases remain lower than possible with single particle analysis cryo-electron microscopy, the contextual insight provided by the tomography approach delivers a unique flavor of insights. Such insights can be crucial for understanding the architecture of filament assemblies (e.g., the actin-rich protrusions of migrating cells) and the role of their organizers. To make the best use of the structural and contextual information contained in tomograms, we assembled workflows and software pipelines that allow for rapid ultrastructural analysis of parallel and meshwork-like filament assemblies, as well as structure determination of proteins organizing such macromolecular constructions.
During this presentation, I will give a short overview of the pipelines and showcase their application, mainly in the context of actin filament networks.
Speaker: Agathe Urvoas, Institute for Integrative Biology of the Cell (I2BC),
Tittle: AlphaRep: a new platform dedicated to the selection of artificial protein binders
May 14, 2024 at 11 am
Summary: We have designed and assembled a large library of artificial repeat proteins named alphaRep. AlphaRep proteins binding tightly and specifically any predefined folded protein can be selected out from this library. AlphaRep generation does not require animal immunization and gives rise to renewable, sequence defined, binders. Specific alphaReps are soluble, efficiently produced, disulfide-free and extremely stable. These artificial proteins can be easily modified (fusion, dimerisation, incorporation of fluorophores, tracers, etc.) and can be used as protein tools in various contexts including Structural Biology and Cellular Biology.
The platform offers generation of alphaReps that bind with high affinity and specificity any chosen protein target. Information and contacts: https://www.i2bc.paris-saclay.fr/structural-biology/alpha-rep/
Please register at: https://us06web.zoom.us/webinar/register/WN_ldZ2FglTSRKoA9_XHvA9aQ
The three national infrastructures ProFi, FranceBioImaging and FRISBI along with the GIS IBiSA are pleased to announce a third call for a funded access to IBiSA-labelled facilities. Our aim is to promote IBiSA facilities networking through transdisciplinary research projects.
Applications should request access to at least two different IBiSA facilities from two disciplines (structural biology, Biological imaging and proteomics, see below a non-exhaustive list). The call is open to any academic laboratory.
For application:
Applications should be submitted to Call-IBISA-FBI-FRISBI-PROFI@i2bc.paris-saclay.fr
using the template document 
Télécharger Template-Call-2023-IBISA-FBI-FRISBI-PROFI.docx (15 KB)
Deadline May 31, 2024
Preparation and biophysical/MS characterization of multiprotein complexes for cryo-EM analysis
The rise in popularity of structural approaches, in particular the advent of the cryo-EM “resolution revolution” has resulted in a dire need for improved strategies for sample production and characterization to prevent congestion in the workflow. This advanced EMBO course will train participants (mainly PhD students and postdocs) with the state-of-the-art approaches for the preparation of large macromolecular assemblies in view of structural studies.
https://meetings.embo.org/event/23-cryo-em
The three national infrastructures ProFi, FranceBioImaging and FRISBI along with the GIS IBiSA
are pleased to announce a second call for a funded access to IBiSA-labelled facilities. Our aim
is to promote IBiSA facilities networking through transdisciplinary research projects.
Applications should request access to at least two different IBiSA facilities from two
disciplines (structural biology, Biological imaging and proteomics, see below a non-exhaustive
list). The call is open to any academic laboratory.
media/Access_call_inter-infra_IBiSA_2023.pdf
This is a workshop distributed over several participating centres of the iNEXT-Discovery infrastructure https://inext-discovery.eu.
Practicals will be run in parallel sessions on each site and shared morning lectures on the background and technical aspects will be followed jointly by all the participants.
It comprises sample vitrification, focused ion beam milling to obtain lamella and cryo-ET data acquisition & evaluation.
If you are interested to join please register here: https://inextfibet.sciencesconf.org/
This call aims to encourage teams to obtain initial access to the services offered by the Research Infrastructures to help their research project.
The objective is to demonstrate the significant impact of these services to improve the quality of their results (resolution, reproducibility, change of scale, etc.) or to help remove technological barriers. We also aim to make our teams aware of the cutting-edge technologies, methods and expertise offered by these national infrastructures.
For more info and application https://www.insb.cnrs.fr/fr/appel-projets-insb-acces-aux-infrastructures-nationales-de-recherche
The RéNaFoBIS network is organising its 10th national school from 1 to 9 June 2023 in Oléron. This school offers theoretical and practical training to the different approaches used in structural biology (diffraction and diffusion of X-rays, NMR, electron microscopy, sample preparations for structural studies, macromolecular interactions. It will focus on the integration of several of these experimental methods to answer the major questions of functional biology at the atomic scale.
https://ecolebios2023.sciencesconf.org/
FRISBI co-finances training in France that is open to national and European structural biology communities.
FRISBI will particularly consider training co-organised with a centre from an another infrastructure written in the “Feuille de route Nationale des Infrastructures” https://www.enseignementsup-recherche.gouv.fr/sites/default/files/2022-03/feuille-de-route-nationale-des-infrastructures-de-recherche---2021-v2--17318.pdf
Deadline for submission: 11 September 2022 for a course held between the 1st of January and 31st of December 2023.
Formular for application 42.3 KB
The 8th FRISBI call for funded access to national structural biology platforms is now open.
Access to FRISBI infrastructure is possible for all scientists working in French laboratories. Scientists working at FRISBI centres cannot request funding for access to their local centre.
· Depending on project size 250-2000 € of support is available as a contribution towards the access costs of each project (consumables, reagents and travel for one person). The number of awards is limited. The user is expected to cover any additional expenses beyond the award price.
· Scientists may visit single or multiple FRISBI platforms for their experiments.
· The deadline for this round of proposal submission is 30 June 2022. Proposals should be submitted via the FRISBI online proposal submission system at http://frisbi.eu/submit-a-proposal/. Once selected, projects should be performed within 12 months.
FRISBI is listed as a National Research Infrastructure in Biology and Health in the new "Feuille de route Nationale des Infrastuctures de Recherche" on the MESRI site
Interdisciplinary access call to Structural biology, Biological imaging and Proteomics facilities
The three national infrastructures ProFi, FranceBioImaging and FRISBI along with the GIS IBiSA are pleased to announce a call for a funded access to IBiSA-labelled facilities. Our aim is to promote IBiSA facilities networking through transdisciplinary research projects.
Applications should request access to at least two different IBiSA facilities from two disciplines (structural biology, Biological imaging and proteomics). The call is open to any academic laboratory.
The amount of the financial support will be up to 5000 € per application to cover facility costs.
Retrouver FRISBI aux Rendez-vous Carnot
https://www.rdv-carnot.com/ et au rendez-vous sur le thème "Research Infrastructures and Health"