FRISBI

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FRENCH INFRASTRUCTURE FOR INTEGRATED STRUCTURAL BIOLOGY

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CBS

IN-HOUSE X-RAY CRISTALLOGRAPHY

A rotating anode X-ray source fitted with two diffractometers adapted to data collection on frozen crystals and on crystallization plates. In the latter case, we take advantage of the recently acquired G-rob plate holder for in situ diffraction recording. This alleviates the risks of crystal cracking which often during mounting in capillaries or in cryoloops, and further deterioration during freezing in liquid nitrogen.

In parallel, the CBS has regular access to the ESRF synchrotron as part of BAG shared with the AFMB (node 4 of FRISBI).

The platform shall offer soon access to fragment screening by mean of X-ray diffraction.

Methodology development:

le Maireet al., Acta D, 2011

Application studies:

de Saint-Jeanet al., J. Cell Biol. 2011.

Labesseet al., N. A. R., 2011.

Gazanionet al., Mol Mic. 2011

Device:

Rigaku MicroMaxTM-007HF generator fitted with Osmic VariMaxTM HF confocal optics and a  CCD detector on the one port connected with the Nat-Xray G-rob robot, and a Osmic Blue confocal optics and a Marresearch mar345 image plate detector on the second port. This generator has a focal spot size of 70µm and a power of 1.2kW leading to a brightness of 3.8 x 109 photons/sec/mm2/mR2.