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FRENCH INFRASTRUCTURE FOR INTEGRATED STRUCTURAL BIOLOGY

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INSTRUCT CENTER - FRANCE 1 - IGBMC

NMR

Liquid state NMR

NMR spectroscopy provides invaluable insights into the structural and dynamical features of biomolecular systems. We are taking advantage of NMR, combined with other structural biology methods, to investigate molecular properties that are important to achieve a given biological function.

Three spectrometers are installed in the lab: a 500 MHz with room temperature probes, a 600 MHz and a 700 MHz with cryo-probes to achieve highest sensitivity.

500 MHz spectrometer: For experiments that don't need best sensitivity and resolution, a 500 MHZ with two room temperature probes is available (a selective proton probe with carbon and nitrogen decoupling and Z gradients;a broadband probe that allows observation of 19F as well as 31P, 13C and 15N with 1H decoupling.

600 MHz spectrometer: For experiments that need high sensitivity and resolution, the 600 MHz spectrometer with cryogenic probe is available (selective proton CryProbeTM with carbon, nitrogen and deuterium decoupling and Z gradients).

700 MHz spectrometer: For experiments that need extreme sensitivity and resolution, the 700 MHZ spectrometer with a selective proton and carbon CryoProbeTM with nitrogen/carbon/deuterium decoupling and Z gradients.

BIBLIOGRAPHY

Production and characterization of a retinoic acid receptor RARγ construction encompassing the DNA binding domain and the disordered N-terminal proline rich domain. Martinez-Zapien D1, Delsuc MA1, Travé G2, Lutzing R1, Rochette-Egly C1, Kieffer B3. Protein Expr Purif. 2014 Mar;95:113-20. doi: 10.1016/j.pep.2013.12.001. Epub 2013 Dec 13.