FRISBI
The methods of X-ray crystallography and NMR spectroscopy can provide detailed information on molecular structure and dynamics. At the cellular level, optical microscopy reveals the spatial distribution and dynamics of molecules tagged with fluorophores. Electron microscopy (EM) overlaps with these approaches, covering a broad range from atomic to cellular structures. The development of cryogenic methods has enabled EM imaging to provide snapshots of biological molecules and cells trapped in a close to native, hydrated state.
The service is equipped with an electron microscope FEI Tecnai T12 operating at 120 kV and equipped with a Gatan K2 camera. Since May 2024, a NEW Glacios 2 200 kV equipped with a Falcon 4i camera coupled to a Selectris X energy filter is able to tackle a large panel of projects dedicated to single particles or cryo-tomography imaging. Complementary equipment for grid preparation and optimization (Leica EM GP and Vitrobot Mark IV) are also available.
For more information see http://www.afmb.univ-mrs.fr/en/facility/structural-biology/electron-microscopy/