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FRENCH INFRASTRUCTURE FOR INTEGRATED STRUCTURAL BIOLOGY

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INSTRUCT CENTER - FRANCE 1 - IGBMC

CRYO ELECTRON MICROSCOPY

The Titan Krios cryo electron microscope is the latest generation electron microscope with capacities for high-resolution data collection for both Cryo Electron Tomography (CET) experiments and Single Particle Analysis (SPA) with automated data collection. The electron source is a Field Emission Gun (FEG) that can be operated at 80keV, 100keV, 200keV or 300keV. An automatic loading mechanism allows mounting twelve grids at a time. This microscope is equipped with CMOS (FEI FALCON3 4K*4K and GATAN K3) high-sensitivity direct electron detector cameras, Cs corrector, phase plate and GIF.

The Glacios cryo-electron microscope allows high-resolution SPA and CET automated data collection. Its FEG is usually tuned to 200keV. An automatic loading mechanism allows mounting twelve grids at a time. This microscope is equipped with two digital cameras: FEI Ceta-D and the direct electron detector GATAN K2

The Tecnai F20 is equipped with a FEG operated at 100keV or 200keV. Grids are mounted one at a time using a Gatan side-entry cryo-holder. This microscope is equipped with one digital camera (GATAN CCD 2K*2K "US10001"), it is used to collect data for cryo-SPA or room temperature electron tomography using a Fischion side-entry holder.

Grid preparation:

For purified samples (not yet on cryo-EM grids) we offer freezing (Vitrobot system) and screening of grids. Cryo-EM grids are flash-frozen in a temperature and humidity controlled environment using a Vitrobot system (FEI).

Although all our microscopes are equipped with digital cameras, we can still record images on films and scan them with a high-resolution Heidelberger Druckmaschinen drum scanner ( 5 micron pixelsize).

The non-infectious character of the samples needs to be confirmed by the director of the applying institute.

Please include a short description of the project and the characterisation of the sample (biophysically characterisation and images / class averages of cryo-EM tests if available). Applications will be handled confidentially as for any applications, submit proposal and select the platform  "cryo electron microscopy (Instruct-centre France 1 IGBMC)"


NOTE: when asking access for Titan or Glacios, please add to your proposal preliminary image of the sample in cryo condition.

See more at https://instruct-eric.eu/platform/electron-microscopy-strasbourg-france/


3rd Instruct-ERIC Best Practices in CryoEM Workshop organised in November 2019

https://instruct-eric.eu/content/3rd-instruct-best-practices-in-cryoem-workshop


BIBLIOGRAPHY