The Titan Krios cryo electron microscope is the latest generation electron microscope with capacities for high-resolution data collection for both Cryo Electron Tomography (CET) experiments and Single Particle Analysis (SPA) with automated data collection. The electron source is a Field Emission Gun (FEG) that can be operated at 80keV, 100keV, 200keV or 300keV. An automatic loading mechanism allows mounting twelve grids at a time. This microscope is equipped with CMOS (FEI FALCON3 4K*4K and GATAN K3) high-sensitivity direct electron detector cameras, Cs corrector, phase plate and GIF.
The Glacios cryo-electron microscope allows high-resolution SPA and CET automated data collection. Its FEG is usually tuned to 200keV. An automatic loading mechanism allows mounting twelve grids at a time. This microscope is equipped with two digital cameras: FEI Ceta-D and the direct electron detector GATAN K2
The Tecnai F20 is equipped with a FEG operated at 100keV or 200keV. Grids are mounted one at a time using a Gatan side-entry cryo-holder. This microscope is equipped with one digital camera (GATAN CCD 2K*2K "US10001"), it is used to collect data for cryo-SPA or room temperature electron tomography using a Fischion side-entry holder.
For purified samples (not yet on cryo-EM grids) we offer freezing (Vitrobot system) and screening of grids. Cryo-EM grids are flash-frozen in a temperature and humidity controlled environment using a Vitrobot system (FEI).
Although all our microscopes are equipped with digital cameras, we can still record images on films and scan them with a high-resolution Heidelberger Druckmaschinen drum scanner ( 5 micron pixelsize).
The non-infectious character of the samples needs to be confirmed by the director of the applying institute.
Please include a short description of the project and the characterisation of the sample (biophysically characterisation and images / class averages of cryo-EM tests if available). Applications will be handled confidentially as for any applications, submit proposal and select the platform "cryo electron microscopy (Instruct-centre France 1 IGBMC)"
NOTE: when asking access for Titan or Glacios, please add to your proposal preliminary image of the sample in cryo condition.
Dynamics of uS19 C-Terminal Tail during the Translation Elongation Cycle in Human Ribosomes. Bhaskar V, Graff-Meyer A, Schenk AD, Cavadini S, von Loeffelholz O, Natchiar SK, Artus-Revel CG, Hotz HR, Bretones G, Klaholz BP, Chao JA. Cell Rep 7 avril 2020;31:107473.
Structure of SAGA and mechanism of TBP deposition on gene promoters. Papai G, Frechard A, Kolesnikova O, Crucifix C, Schultz P, Ben-Shem A. Nature Jan 2020 ; 577:711-716 .
Targeting the Human 80S Ribosome in Cancer: From Structure to Function and Drug Design for Innovative Adjuvant Therapeutic Strategies. Gilles A, Frechin L, Natchiar K, Biondani G, Loeffelholz OV, Holvec S, Malaval JL, Winum JY, Klaholz BP, Peyron J. Cells 5 mars 2020.
Activation of the Endonuclease that Defines mRNA 3' Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex. Hill CH, Boreikaite V, Kumar A, Casanal A, Kubik P, Degliesposti G, Maslen S, Mariani A, von Loeffelholz O, Girbig M, Skehel M, Passmore LA. Mol Cell 21 mars 2019 ; 73:1217-1231
Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex. Vanden Broeck A, Lotz C), Ortiz J, Lamour V. Nat Commun 30 octobre 2019 ; 10:4935
Structural Basis of Transcription: RNA Polymerase Backtracking and Its Reactivation. Abdelkareem M, Saint-Andre C, Takacs M, Papai G, Crucifix C, Guo X, Ortiz J, Weixlbaumer A.Mol Cell 8 mai 2019
Structural Basis of Transcription: RNA Polymerase Backtracking and Its Reactivation. Abdelkareem M, Saint-Andre C, Takacs M, Papai G, Crucifix C, Guo X, Ortiz J, Weixlbaumer A .Mol Cell 25 juillet 2019 ; 75:298-309
The CryoEM structure of the Saccharomyces cerevisiae ribosome maturation factor Rea1. Sosnowski P, Urnavicius L, Boland A, Fagiewicz R, Busselez J, Papai G, Schmidt H. Elife 21 novembre 2018 .
Molecular structure of promoter-bound yeast TFIID. Kolesnikova O, Ben-Shem A, Luo J, Ranish J, Schultz P, Papai G.Nat Commun 7 novembre 2018 ; 9:4666 .
Visualization of chemical modifications in the human 80S ribosome structure. Natchiar SK, Myasnikov AG, Kratzat H, Hazemann I, Klaholz BP. Nature 23 novembre 2017;551:472-477
Structure of the human 80S ribosome. Khatter H, Myasnikov AG, Natchiar SK, Klaholz BP Nature 30 avril 2015;520:640-5.