FIDA combines Taylor dispersion analysis (TDA) and fluorescence detection in straight fused silica capillaries to measure biomolecular size, enabling accurate determination of hydrodynamic radius (Rh) and size-based characterization of biomolecular interactions. Species of Rh between 0.5 and 1000 nm can be analyzed, and different populations resolved. Size changes down to 5% hydrodynamic radius can be detected. For biomolecular complex characterization, measurements at different concentrations of analyte (= non-fluorescent binding partner) and constant concentration of the indicator (= fluorescent biomolecule) result in a binding curve from which the dissociation constant Kd and Rh of indicator and complex are obtained. Measurements using Fida1 can be performed in plasma, serum, cell lysate and fermentation media.
Device: Fida1 (Fidabio) with 480 nm ex LED fluorescence detection