Liquid state NMR
NMR spectroscopy provides invaluable insights into the structural and dynamical features of biomolecular systems. We are taking advantage of NMR, combined with other structural biology methods, to investigate molecular properties that are important to achieve a given biological function.
Two spectrometers are installed in the lab; a 600 MHz and a 700 MHz with cryo-probes to achieve highest sensitivity.
600 MHz spectrometer: For experiments that need high sensitivity and resolution, the 600 MHz spectrometer with cryogenic probe is available (selective proton CryProbeTM with carbon, nitrogen and deuterium decoupling and Z gradients).
700 MHz spectrometer: For experiments that need extreme sensitivity and resolution, the 700 MHZ spectrometer with a selective proton and carbon CryoProbeTM with nitrogen/carbon/deuterium decoupling and Z gradients.
Production and characterization of a retinoic acid receptor RARγ construction encompassing the DNA binding domain and the disordered N-terminal proline rich domain. Martinez-Zapien D1, Delsuc MA1, Travé G2, Lutzing R1, Rochette-Egly C1, Kieffer B3. Protein Expr Purif. 2014 Mar;95:113-20. doi: 10.1016/j.pep.2013.12.001. Epub 2013 Dec 13.