ANALYTICAL ULTRA CENTRIFUGATION

Analytical ultracentrifugation is a technique using centrifugal force to study the sedimentation of macromolecules in solution. Thus, the behavior of a multitude of molecules can be studied at a wide concentration range and in a wide variety of buffers. This technique, allow to study shape and size of species ranging from the peptide to the capsid or the vesicle by way of proteins, but also allows to study properties of various complexes between proteins, detergent, RNA, DNA, polymer, glycosylated protein ... . PIM platform is equipped with an analytical ultracentrifuge XL-I (Absorbance and interference detector) to which is added a fluorescence detector. By using this equipment and two different approaches (sedimentation equilibrium and sedimentation velocity) various type of information can be obtained:

• Association constants, Kd (large range of Kd from pM to µM)
• Sample homogeneity
• Molecular weight
• Stoichiometry of multi-component systems.

See more at:

http://www.i2bc.paris-saclay.fr/spip.php?article331&lang=en

Bibliography :

The UbiK protein is an accessory factor necessary for bacterial ubiquinone (UQ) biosynthesis and forms a complex with the UQ biogenesis factor UbiJ. Loiseau L, Fyfe C, Aussel L, Hajj Chehade M, Hernández SB, Faivre B, Hamdane D, Mellot-Draznieks C, Rascalou B, Pelosi L, Velours C, Cornu D, Lombard M, Casadesús J, Pierrel F, Fontecave M, Barras F.