MALS

Size exclusion chromatography (SEC) coupled with multiangle light scattering (MALS) is a straightforward technique to determine the accurate molar mass and the average size of proteins and macromolecular complexes in solution. Indeed, MALS can measure the absolute molar mass and size of molecules without the use of reference standards.

One of the major application is the determination of the size and stoichiometry of tightly bound heterocomplexes, such as protein/protein, protein/DNA, protein/RNA and protein/detergent interactions.

System components:

• Multi-angle light scattering detector: miniDAWN TREOS from Wyatt Technology. Equipped with the QELS module which enables measurement of the hydrodynamic radius.
• Differential Refractive Index (dRI) detector: Optilab T-rEX from Wyatt Technology.
• Chromatography system: Ettan MicroLC from GE Healthcare.