ANALYTICAL ULTRA CENTRIFUGATION

Analytical ultracentrifugation (AUC) studies macromolecules in solution that are subjected to a centrifugal field. AUC combines the separation of the macromolecules and the analysis of their transportation in view of a rigorous thermodynamics. AUC is particularly suitable for studying the interactions of membrane proteins, glycosylated proteins, complexes between proteins and polymer or ANR... Sedimentation velocity and sedimentation equilibrium experiments allow :

•  assessing sample homogeneity
•  determining the association state of complexes
•  providing indications on the general shape of the macromolecules
•  quantifying association constants and weak inter-molecular interactions that determine protein solubility.
•  analysing complex multi-component systems, on the basis of their density and optical properties (absorbance and refractive index).

See more at:

http://www.isbg.fr/biophysics-characterisation/analytical-ultracentrifugation-auc/

https://instruct-eric.eu/platform/molecular-biophysics-grenoble-france/