FRISBI
FRISBI's centres offer a complete set of platforms (techniques) for the integrated structural biology research process. Explore the platforms by stage of the process.
Sample preparation is not only a key issue for structural and molecular biology studies, but concerns Life Sciences development as a whole. FRISBI offer state-of-the-art infrastructures for the expression and production of biomolecules and their complexes in prokaryotic and eukaryotic system.
Bacterial hosts are hosts of choice for the production of recombinant proteins and protein complexes. This is largely due to the ease of genetic manipulation and simple culturing.
FRISBI centers offering this: Instruct Center France 1-IGBMC, Instruct Center France 2-IBS-ISBG ; AFMB Marseille
Yeast cells constitute a convenient alternative host for production of either recombinant eukaryotic proteins or endogenous complexes. Advantages include the low cost of growing material, robustness of the system, easy genetic manipulation, and the presence of endogenous eukaryotic-type protein modification and chaperone systems
FRISBI centers offering this: Instruct Centre France 1-IGBMC; Paris Saclay
Baculovirus expression vector system (BEVS) is a powerful and polyvalent tool for recombinant protein expression. Without any danger known for human beings, recombinant baculoviruses allow us to produce a large amount of proteins and protein complexes that are difficult to obtain with other biological systems.
FRISBI centers offering this: Instruct Center France1-IGBMC; I AFMB; Paris Saclay
Mammalian expression systems are more and more the method of choice for many structural biology projects where correct folding machinery or post translational modifications are decisively important.
FRISBI centers offering this: Instruct Centre France 1-IGBMC, AFMB
FRISBI centers offering this: Instruct Centre France 1-IGBMC
Cell free (CF) expression systems based on cell extracts (E.coli or wheat germ) have shown to be very effective for the production of soluble proteins and in particular for membrane proteins. In addition, CF expression offers unique opportunities for the efficient and fast labelling of proteins with isotopes or modified amini acids.
FRISBI centers offering this: Instruct Centre France 2-IBS-ISBG
FRISBI centers offering this: CBS
FRISBI centers offering this: Instruct Center France 2-IBS-ISBG
FRISBI centers offering this: Instruct Center France 1-IGBMC, CBS
Mass spectrometry
FRISBI centers offering this: Instruct Center France 2-IBS-ISGB
FRISBI centers offering this: AFMB
FRISBI offers a large panel of state-of-the-art equipment and expertise in biochemistry and biophysics to perform quality control and functional analysis on protein samples.
FRISBI centers offering this: Instruct Center France 1-IGBMC; Instruct Center France 2-IBS-ISBG; Paris Saclay
FRISBI centers offering this: Instruct Center France 1-IGBMC; AFMB; Paris Saclay
FRISBI centers offering this: Instruct Center France 1-IGBMC; AFMB; CBS; Paris Saclay
FRISBI centers offering this: Instruct Center France 1-IGBMC; CBS
FRISBI centers offering this:Instruct Center France 1-IGBMC
FRISBI centers offering this:
FRISBI centers offering this:Instruct Center France 1-IGBMC; Instruct Center France 2-IBS-ISBG; AFMB
FRISBI centers offering this: AFMB; Paris Saclay; Instruct Centre France 1-IGBMC
FRISBI centers offering this: Paris Saclay
FRISBI centers offering this: Instruct Center France 2-IBS-ISBG ;
FRISBI centers offering this: Paris Saclay
FRISBI centers offering this: Instruct Center France 1 -IGBMC; CBS; AFMB
Crystallisation of protein molecule is a crucial step during the course of X-ray structure determination.
FRISBI centers offering this:Instruct Center France 2-IBS-ISBG
FRISBI centers offering this: Instruct Center France 1-IGBMC; Instruct Center France 2-IBS-ISBG; Paris Saclay, CBS, AFMB
FRISBI centers offering this: Instruct Center France 2-IBS-ISBG
FRISBI offers acces to a range of state of the art structural biology technologies and integrative biological approaches and expertise.
FRISBI centers offering this: Instruct Center France 1-IGBMC; Instruct Center France 2 IBS-ISBG; AFMB; CBS; Paris Saclay
FRISBI centers offering this: Instruct Center France 1-IGBMC; Instruct Center France 2-IBS-ISBG
FRISBI centers offering this: Instruct Center France 1-IGBMC
FRISBI centers offering this: Instruct Centre France 1-IGBMC; Instruct Centre France 2-IBS-ISBG; Paris Saclay, CBS
FRISBI centers offering this: Instruct Center France 2-IBS-ISBG
FRISBI centers offering this: Paris Saclay
FRISBI centers offering this: Paris Saclay
FRISBI centers offering this: Paris Saclay
FRISBI centers offering this: Paris Saclay
FRISBI centers offering this: Paris Saclay
FRISBI centers offering this: CBS
FRISBI centers offering this: AFMB, CBS
FRISBI centers offering this: Instruct Center France 2- IBS-ISBG
FRISBI centers offering this: Paris Saclay
In the framework of integrated structural biology, the goal of bioinformatics is not only to facilitate the determination of a protein’s 3D structure, but also to understand its role(s) in the complex, dynamic networks that are responsible for cellular or organismal functions. This requires dedicated computational systems that can handle and store large volumes of heterogeneous data and that can quickly process queries between the different data resources and efficiently extract relevant knowledge.